Abstract:

BACKGROUND: Keloid formation is affected by regulations of many genes and cytokines. However, it is not clear about the mechanism underlying the regulation of these genes and cytokines expressions.
OBJECTIVE: To investigate the differential expression of microRNAs between human normal skins and keloid tissues, and to initially screen microRNAs expression profiling in keloid tissues.
METHODS: According to clinical diagnostic criteria and experiment demands, the inclusion criteria and exclusion criteria of keloids and normal skins were formulated: keloids (six cases) and normal human skins (five cases). Total RNAs were extracted from keloids and normal skin tissues by Trizol, and microRNAs were further isolated by Ambion’s miRNA Isolation Kit. In keloids, the differential expression profile of microRNAs was harvested by significance analysis of microarrays (SAM) and Cluster, based on microarray screening. Real time quantitative reverse transcription-PCR was applied to verify reliability of microRNA array results.
RESULTS AND CONCLUSION: Differential expression profile of MicroRNAs (n=12) was obtained in human keloids, which may be related to the pathogenesis and development of keloids. Moreover, part of them may be used for unique molecular markers for diagnosis and treatment of keloids.